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pCMV-Tag4/hL3MBTL1
(Plasmid #28231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-Tag4A
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    l(3)mbt-like 1 (Drosophila)
  • Alt name
    L3MBTL1
  • Alt name
    L3MBTL
  • Alt name
    H-L(3)MBT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2319
  • Mutation
    Irrelevant S49T mutation
  • GenBank ID
    NM_015478
  • Entrez Gene
    L3MBTL1 (a.k.a. H-L(3)MBT, L3MBTL, ZC2HC3, dJ138B7.3)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVPro Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag4/hL3MBTL1 was a gift from Danny Reinberg (Addgene plasmid # 28231 ; http://n2t.net/addgene:28231 ; RRID:Addgene_28231)

  • For your References section:

    L3MBTL1, a histone-methylation-dependent chromatin lock. Trojer P, Li G, Sims RJ, Vaquero A, Kalakonda N, Boccuni P, Lee D, Erdjument-Bromage H, Tempst P, Nimer SD, Wang YH, Reinberg D. Cell. 2007 Jun 1. 129(5):915-28. 10.1016/j.cell.2007.03.048 PubMed 17540172
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