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Addgene

p-LCN2
(Plasmid #28225)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28225 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4817
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LCN2 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1233
  • GenBank ID
  • Entrez Gene
    LCN2 (a.k.a. 24p3, MSFI, NGAL, p25)
  • Promoter LCN2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer REVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    human promoter cloned by the provider
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-LCN2 was a gift from Sebastien Jauliac (Addgene plasmid # 28225 ; http://n2t.net/addgene:28225 ; RRID:Addgene_28225)

  • For your References section:

    NFAT3 transcription factor inhibits breast cancer cell motility by targeting the Lipocalin 2 gene. Fougere M, Gaudineau B, Barbier J, Guaddachi F, Feugeas JP, Auboeuf D, Jauliac S. Oncogene. 2010 Apr 15. 29(15):2292-301. 10.1038/onc.2009.499 PubMed 20101218
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