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Addgene

pcDNA3.T7 NFAT3
(Plasmid #28223)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28223 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5469
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human NFAT3
  • Alt name
    NFATC4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2715
  • Mutation
    G160A
  • GenBank ID
    NM_004554
  • Entrez Gene
    NFATC4 (a.k.a. NF-AT3, NF-ATC4, NFAT3)
  • Promoter CMV
  • Tag / Fusion Protein
    • T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tim Hoey

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a G160A mutation in the NFAT3 sequence when compared to GenBank reference NP_004545.2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.T7 NFAT3 was a gift from Sebastien Jauliac (Addgene plasmid # 28223 ; http://n2t.net/addgene:28223 ; RRID:Addgene_28223)

  • For your References section:

    NFAT3 transcription factor inhibits breast cancer cell motility by targeting the Lipocalin 2 gene. Fougere M, Gaudineau B, Barbier J, Guaddachi F, Feugeas JP, Auboeuf D, Jauliac S. Oncogene. 2010 Apr 15. 29(15):2292-301. 10.1038/onc.2009.499 PubMed 20101218
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