SI1-Unc5b
(Plasmid #28214)

• Depositing Lab: Gavin Wright
• Publication: Crosnier et al BMC Biol. 2010 . 8():76.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 5925
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Unc5b mouse monoclonal antibody
• Alt name: Unc5b
• Species: D. rerio (zebrafish)
• Entrez Gene: unc5b (a.k.a. unc5h2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13_puc_Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See map and Genbank file for plasmid details.

Please note that Addgene's sequencing results identified 4 single nucleotide mismatches (at bp# 3373, 3376, 3380 and 3386) when compared to the full plasmid sequence. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.