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Addgene

pCR.MCV.Ori339 (589)
(Plasmid #28192)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR2.1-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCV replication region
  • Alt name
    Human Merkel Cell polyomavirus
  • Species
    Human Merkel Cell polyomavirus
  • Insert Size (bp)
    583

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer M13pUC-fwd
  • 3′ sequencing primer M13rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR.MCV.Ori339 (589) was a gift from Patrick Moore (Addgene plasmid # 28192 ; http://n2t.net/addgene:28192 ; RRID:Addgene_28192)

  • For your References section:

    T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus. Shuda M, Feng H, Kwun HJ, Rosen ST, Gjoerup O, Moore PS, Chang Y. Proc Natl Acad Sci U S A. 2008 Oct 21. 105(42):16272-7. 10.1073/pnas.0806526105 PubMed 18812503
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