p-1459-adcy4-Luc
(Plasmid #28184)

• Depositing Lab: Bernard Schimmer
• Publication: Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Adcy4 promoter
• Alt name: Adcy4
• Species: M. musculus (mouse)
• Insert Size (bp): 1812
• Entrez Gene: Adcy4 (a.k.a. KIAA4004, mKIAA4004)
• Tag / Fusion Protein:
  • Luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: Luc_N_Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a total of ~1812 bp of genomic DNA--i.e.,1459 bp upstream of the Adcy4 transcription start site and 353 bp downstream of the transcription start site. The downstream DNA includes exon I, intron I and the non-coding portion of exon II.