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His-tagged-SF1 (antisense)
(Plasmid #28183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-his
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SF1
  • Alt name
    Nr5a1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2022
  • Mutation
    Reverse Orientation
  • Entrez Gene
    Nr5a1 (a.k.a. Ad4BP, ELP, ELP-3, Ftz-F1, Ftzf1, SF-1, SF1, STF-1)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Keith L. Parker, Duke Univ.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The His-tagged-SF1 (antisense) plasmid, the plasmid was created as a control for the His-tagged-SF1 plasmid (#28182). The SF1 insert was placed in the 3' - 5' orientation downstream of the His tag. As such, the transcript will not encode an open reading frame.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-tagged-SF1 (antisense) was a gift from Bernard Schimmer (Addgene plasmid # 28183 ; http://n2t.net/addgene:28183 ; RRID:Addgene_28183)

  • For your References section:

    Impaired steroidogenic factor 1 (NR5A1) activity in mutant Y1 mouse adrenocortical tumor cells. Frigeri C, Tsao J, Czerwinski W, Schimmer BP. Mol Endocrinol. 2000 Apr . 14(4):535-44. 10.1210/mend.14.4.0440 PubMed 10770490
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