GFP-Nucleolin
(Plasmid #28176)

• Depositing Lab: Michael Kastan
• Publication: Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Backbone

• Vector backbone: pEGFPC1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nucleolin
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Entrez Gene: NCL (a.k.a. C23, Nsr1)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: France Carrier
• Articles Citing this Plasmid:
  • 19 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There are a few mutations (K132R, K141E, & K142E and G680S) in the nucleolin but they are not thought to affect function.