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Addgene

145-pGL3ctrl-3' UTR
(Plasmid #28175)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28175 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-control vector
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5256
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Luciferase flanked by tp53 UTRs
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1352
  • Mutation
    Note that insert length is based on sum of the 2 UTRs (145+1207)
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII/NcoI (5'UTR) (not destroyed)
  • 3′ cloning site XbaI (3'UTR) (not destroyed)
  • 5′ sequencing primer pGL3ctrl luc spb1
  • 3′ sequencing primer pGL3 spb2
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    145-pGL3ctrl-3' UTR was a gift from Michael Kastan (Addgene plasmid # 28175 ; http://n2t.net/addgene:28175 ; RRID:Addgene_28175)

  • For your References section:

    5'-3'-UTR interactions regulate p53 mRNA translation and provide a target for modulating p53 induction after DNA damage. Chen J, Kastan MB. Genes Dev. 2010 Oct 1. 24(19):2146-56. 10.1101/gad.1968910 PubMed 20837656
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