pET283xFlagPP7CPHis
(Plasmid #28174)

• Depositing Lab: Kathleen Collins
• Publication: Collins lab PP7CP plasmids (unpublished)

Backbone

• Vector backbone: pET28
• Backbone size w/o insert (bp): 5300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PP7 coat protein optimized
• Species: PP7 phage modified
• Insert Size (bp): 450
• Mutation: C68A,C73A
• Tags / Fusion Proteins:
  • triple FLAG peptide (N terminal on insert)
  • 6xhistidine (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: T7 Fwd

Resource Information

• Supplemental Documents:
  • Coat Protein Purification Protocol
• A portion of this plasmid was derived from a plasmid made by: David Peabody
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry