pET28HistevTtTERT(1-516)
(Plasmid
#28171)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28
- Backbone size w/o insert (bp) 5300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTetrahymena TERT
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Speciessynthetic gene fragment
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Insert Size (bp)1550
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Tag
/ Fusion Protein
- 6xhistidine (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28HistevTtTERT(1-516) was a gift from Kathleen Collins (Addgene plasmid # 28171 ; http://n2t.net/addgene:28171 ; RRID:Addgene_28171) -
For your References section:
Two purified domains of telomerase reverse transcriptase reconstitute sequence-specific interactions with RNA. O'Connor CM, Lai CK, Collins K. J Biol Chem. 2005 Apr 29. 280(17):17533-9. 10.1074/jbc.M501211200 PubMed 15731105