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Addgene

pBS U3-hTR-500 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 28170 with BamHI-HF and SalI-HF.

We expected and saw the following banding patterns:

BamHI-HF single digest: 1 band: 4.3kb

BamHI-HF / SalI-HF double digest:2 bands: 3 and 1.3kb