pBS U3-hTR-500
(Plasmid #28170)

• Depositing Lab: Kathleen Collins
• Publication: Fu et al Mol Cell. 2003 May . 11(5):1361-72.

Backbone

• Vector backbone: pBS
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U3-hTR-500
• Alt name: human telomerase RNA
• Alt name: TER
• Alt name: TERC
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Entrez Gene: TERC (a.k.a. DKCA1, PFBMFT2, SCARNA19, TER, TR, TRC3, hTR)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that BLAST results bring back one T->C nt change in the TERC, but this is the plasmid used in the associated article.