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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 28169 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABEhygro
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression, Retroviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameeGFPhTERT
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SpeciesH. sapiens (human); eGFP
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Insert Size (bp)4250
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABEhygroHigheGFPhTERT was a gift from Kathleen Collins (Addgene plasmid # 28169 ; http://n2t.net/addgene:28169 ; RRID:Addgene_28169) -
For your References section:
Subnuclear shuttling of human telomerase induced by transformation and DNA damage. Wong JM, Kusdra L, Collins K. Nat Cell Biol. 2002 Sep . 4(9):731-6. 10.1038/ncb846 PubMed 12198499