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Addgene

EPHB3
(Plasmid #28165)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28165 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFHMSP-LIC-N (Addgene plasmid 26095)
  • Backbone manufacturer
    SGC
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EPHB3
  • Alt name
    PDB: 3P1I
  • Alt name
    EPH receptor B3, ligand binding domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    519
  • Mutation
    Ligand binding domain (residues 39-211).
  • Entrez Gene
    EPHB3 (a.k.a. EK2, ETK2, HEK2, TYRO6)
  • Tags / Fusion Proteins
    • Honeybee melittin Signal sequence (N terminal on backbone)
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer polyhedrin-fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EPHB3 was a gift from Cheryl Arrowsmith (Addgene plasmid # 28165 ; http://n2t.net/addgene:28165 ; RRID:Addgene_28165)
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