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Addgene

P100 (3OMC)
(Plasmid #28159)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28159 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28-MHL (Addgene plasmid 26096)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7325
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SND1
  • Alt name
    PDB: 3OMC, 3OMG
  • Alt name
    Staphylococcal nuclease domain-containing protein 1, SND1 Tudor domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • Mutation
    Tudor domain (residues 650-910).
  • Entrez Gene
    SND1 (a.k.a. TDRD11, TSN, Tudor-SN, p100)
  • Tag / Fusion Protein
    • His tag with TEV cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P100 (3OMC) was a gift from Cheryl Arrowsmith (Addgene plasmid # 28159 ; http://n2t.net/addgene:28159 ; RRID:Addgene_28159)
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