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Addgene

HLTF
(Plasmid #28152)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 28152 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pET28a-LIC (Addgene plasmid 26094)
  • Backbone manufacturer
    SGC
  • Backbone size w/o insert (bp) 7328
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    helicase-like transcription factor
  • Alt name
    PDB: 2L1I
  • Alt name
    helicase-like transcription factor, HIRAN domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    360
  • Mutation
    Contains HIRAN domain (residues 51 to 171).
  • Entrez Gene
    HLTF (a.k.a. HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1)
  • Tag / Fusion Protein
    • thrombin cleavage (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ligation-independent cloning (unknown if destroyed)
  • 3′ cloning site ligation-independent cloning (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HLTF was a gift from Cheryl Arrowsmith (Addgene plasmid # 28152 ; http://n2t.net/addgene:28152 ; RRID:Addgene_28152)
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