GST-TRIM24ΔRING
(Plasmid
#28148)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDEST15
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRIM24
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SpeciesH. sapiens (human)
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MutationDeletion of the RING domain: aa 1-139
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Entrez GeneTRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attR1 (not destroyed)
- 3′ cloning site attR2 (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-TRIM24ΔRING was a gift from Michelle Barton (Addgene plasmid # 28148 ; http://n2t.net/addgene:28148 ; RRID:Addgene_28148)