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Addgene

Flag-TRIM24-C840W shRescue
(Plasmid #28137)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28137 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMX-(Flag)x2
  • Backbone manufacturer
    Barton Lab
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRIM24
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3200
  • Mutation
    C840Wmutated at aa 308 and 309 for shRNA rescue; A489V
  • Entrez Gene
    TRIM24 (a.k.a. PTC6, RNF82, TF1A, TIF1, TIF1A, TIF1ALPHA, hTIF1)
  • Tag / Fusion Protein
    • FLAGX2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer 5-GTCACACCACAGAAGTAAGGT-3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results identified a A489V mutation in the TRIM24 sequence. This mutation was not intentional and the depositing laboratory states that it is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-TRIM24-C840W shRescue was a gift from Michelle Barton (Addgene plasmid # 28137 ; http://n2t.net/addgene:28137 ; RRID:Addgene_28137)

  • For your References section:

    TRIM24 links a non-canonical histone signature to breast cancer. Tsai WW, Wang Z, Yiu TT, Akdemir KC, Xia W, Winter S, Tsai CY, Shi X, Schwarzer D, Plunkett W, Aronow B, Gozani O, Fischle W, Hung MC, Patel DJ, Barton MC. Nature. 2010 Dec 16. 468(7326):927-32. 10.1038/nature09542 PubMed 21164480
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