FLX1.8MitoKaede
(Plasmid #28133)

• Depositing Lab: Geoffrey Owens
• Publication: Owens lab FIV plasmids (unpublished)

Backbone

• Vector backbone: FLX1.8CMV
• Backbone size w/o insert (bp): 5652
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MitoKaede
• Species: Trachyphyllia geoffroyi
• Insert Size (bp): 782
• GenBank ID: AB085641
• Tag / Fusion Protein:
  • COX VIII signal sequence (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: MBL International Corporation
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene provides this plasmid in Stbl3, but the depositing scientist recommends using MDS42recA
from www.scarabgenomics.com for further amplification.