FLX1.8MitoKate2
(Plasmid
#28132)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28132 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFLX1.8CMV
- Backbone size w/o insert (bp) 5652
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMitoKate2
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SpeciesEntacmaea quadricolor
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Insert Size (bp)806
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GenBank IDGV616184
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Tag
/ Fusion Protein
- COX VIII signal sequence (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site Sal I (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEvrogen
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene provides this plasmid in Stbl3, but the depositing scientist recommends using MDS42recA
from www.scarabgenomics.com for further amplification.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLX1.8MitoKate2 was a gift from Geoffrey Owens (Addgene plasmid # 28132 ; http://n2t.net/addgene:28132 ; RRID:Addgene_28132)
Map uploaded by the depositor.
