Myc-Cdh1
(Plasmid
#28127)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCV-puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6300
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Vector typeMammalian Expression, Mouse Targeting, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namefzr1
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Alt nameHct1, srw1, cdh1
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Alt namefizzy-related 1
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SpeciesH. sapiens (human)
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GenBank IDNP_057347.2
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Entrez GeneFZR1 (a.k.a. CDC20C, CDH1, DEE109, FZR, FZR2, HCDH, HCDH1)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: this plasmid encodes for the fizzy-related 1 gene and NOT E-cadherin.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Myc-Cdh1 was a gift from Pumin Zhang (Addgene plasmid # 28127 ; http://n2t.net/addgene:28127 ; RRID:Addgene_28127) -
For your References section:
The adaptor protein of the anaphase promoting complex Cdh1 is essential in maintaining replicative lifespan and in learning and memory. Li M, Shin YH, Hou L, Huang X, Wei Z, Klann E, Zhang P. Nat Cell Biol. 2008 Sep . 10(9):1083-9. 10.1038/ncb1768 PubMed 19160489