USP08 (3N3K)
(Plasmid #28115)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q2 (unpublished)

Backbone

• Vector backbone: pET28a-LIC (Addgene plasmid 26094)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: USP08
• Alt name: PDB: 3N3K, 2GFO
• Alt name: ubiquitin specific protease 8
• Species: H. sapiens (human)
• Insert Size (bp): 1131
• Mutation: Catalytic domain only.
• Entrez Gene: USP8 (a.k.a. HumORF8, PITA4, SPG59, UBPY)
• Tag / Fusion Protein:
  • His tag with TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB:
3N3K http://www.thesgc.org/structures/3N3K/
2GFO http://www.thesgc.org/structures/2GFO/

This construct is identical to 2GFO