SUV39H1
(Plasmid #28113)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q2 (unpublished)

Backbone

• Vector backbone: pET28-MHL (Addgene plasmid 26096)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SUV39H1
• Alt name: PDB: 3MTS
• Alt name: suppressor of variegation 3-9 homolog 1, chromodomain
• Species: H. sapiens (human)
• Insert Size (bp): 189
• Mutation: contains chromo domain only (amino acid residues 44-106).
• Entrez Gene: SUV39H1 (a.k.a. H3-K9-HMTase 1, KMT1A, MG44, SUV39H)
• Tag / Fusion Protein:
  • His tag with TEV site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB 3MTS: http://www.thesgc.org/structures/3MTS/