USP21 (3MTN)
(Plasmid #28112)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q2 (unpublished)

Backbone

• Vector backbone: pET28a-LIC (Addgene plasmid 26094)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: USP21
• Alt name: PDB: 3MTN
• Alt name: ubiquitin specific peptidase 21
• Species: H. sapiens (human)
• Insert Size (bp): 1062
• Mutation: catalytic domain only (amino acid residues 209-562).
• Entrez Gene: USP21 (a.k.a. USP16, USP23)
• Tag / Fusion Protein:
  • His tag with Thrombin cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3MTN. http://www.thesgc.org/structures/3MTN/