BRPF1 (3MO8)
(Plasmid #28107)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q2 (unpublished)

Backbone

• Vector backbone: pET28-MHL (Addgene plasmid 26096)
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BRPF1:JMC014-E01:C44383
• Alt name: PDB IDs: 3L42, 3MO8, 5C6S
• Alt name: bromodomain and PHD finger containing 1, PWWP domain
• Alt name: gi|51173719
• Species: H. sapiens (human)
• Insert Size (bp): 387
• Entrez Gene: BRPF1 (a.k.a. BR140, IDDDFP)
• Tag / Fusion Protein:
  • His-tag with integrated TEV protease site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 3′ cloning site: Ligation-independent cloning (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3MO8. https://www.thesgc.org/structures/3MO8/
PDB: 5C6S. https://www.thesgc.org/structures/5C6S

Note that this clone is the same as PDB IDs 3MO8 and 5C6S