hWIP1 D314A FLAG
(Plasmid #28106)

• Depositing Lab: Lawrence Donehower
• Publication: Moon et al J Biol Chem. 2010 Apr 23. 285(17):12935-47.

Backbone

• Vector backbone: pCMV-Neo-Bam
• Backbone size w/o insert (bp): 7767
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: WIP1
• Alt name: wild type p53 induced phosphatase
• Alt name: PPM1D
• Alt name: protein phosphatase magnesium-dependent 1 delta
• Species: H. sapiens (human)
• Insert Size (bp): 1865
• Mutation: Contains phosphatase-dead mutation D314A
• Entrez Gene: PPM1D (a.k.a. IDDGIP, JDVS, PP2C-DELTA, WIP1)
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry