pGL3-PTEN 3'UTR
(Plasmid #28104)

• Depositing Lab: Jeffrey Rosen
• Publication: Greene et al J Cell Sci. 2010 Feb 15. 123(Pt 4):606-18.

Backbone

• Vector backbone: pGL3-Promoter
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5010
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN 3'UTR
• Alt name: phosphatase and tensin homolog
• Species: M. musculus (mouse)
• Insert Size (bp): 2700
• Entrez Gene: Pten (a.k.a. 2310035O07Rik, A130070J02Rik, B430203M17Rik, MMAC1, PTENbeta, TEP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that Addgene's sequencing result contains a 3 nucleotide deletion (bp# 7639-7641) when compared with GenBank accession number NM_008960.2

PTEN 3'UTR was amplified from Balb/c mouse genomic DNA with the following primer pairs:
F: 5' GCTAGCCCCCCTCCTTG
R: 5' GCTAGCAAAAATAATGAACCTTTTTAATTGTTTAAAGG

PCR products were digested with NheI and subcloned into the XbaI site of the pGL3-promoter vector (Promega)