FLAG-Cdk9 T186A
(Plasmid
#28101)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdk9
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Alt nameCdk9-42k
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SpeciesH. sapiens (human)
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Insert Size (bp)1057
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MutationContains a T186A mutation in Cdk9
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Entrez GeneCDK9 (a.k.a. C-2k, CDC2L4, CTK1, PITALRE, TAK)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses the Cdk9 42-kDa isoform
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FLAG-Cdk9 T186A was a gift from Andrew Rice (Addgene plasmid # 28101 ; http://n2t.net/addgene:28101 ; RRID:Addgene_28101) -
For your References section:
Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 T-loop. Wang Y, Dow EC, Liang YY, Ramakrishnan R, Liu H, Sung TL, Lin X, Rice AP. J Biol Chem. 2008 Nov 28. 283(48):33578-84. 10.1074/jbc.M807495200 PubMed 18829461
Map uploaded by the depositor.
