Skip to main content
Addgene

FLAG-Cdk9
(Plasmid #28100)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFLAG-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cdk9
  • Alt name
    Cdk9-42k
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1057
  • Entrez Gene
    CDK9 (a.k.a. C-2k, CDC2L4, CTK1, PITALRE, TAK)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses the Cdk9 42-kDa isoform

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Cdk9 was a gift from Andrew Rice (Addgene plasmid # 28100 ; http://n2t.net/addgene:28100 ; RRID:Addgene_28100)

  • For your References section:

    Phosphatase PPM1A regulates phosphorylation of Thr-186 in the Cdk9 T-loop. Wang Y, Dow EC, Liang YY, Ramakrishnan R, Liu H, Sung TL, Lin X, Rice AP. J Biol Chem. 2008 Nov 28. 283(48):33578-84. 10.1074/jbc.M807495200 PubMed 18829461
Related items:
Commonly requested with: