pBABE-Flag-Cdk9-T186A-IRES-eGFP
(Plasmid
#28097)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28097 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBMN-I-GFP
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdk9
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SpeciesH. sapiens (human)
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Insert Size (bp)7500
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MutationContains T186A mutation in Cdk9
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Entrez GeneCDK9 (a.k.a. C-2k, CDC2L4, CTK1, PITALRE, TAK)
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBABE-Flag-Cdk9-T186A-IRES-eGFP was a gift from Andrew Rice (Addgene plasmid # 28097 ; http://n2t.net/addgene:28097 ; RRID:Addgene_28097) -
For your References section:
T-loop phosphorylated Cdk9 localizes to nuclear speckle domains which may serve as sites of active P-TEFb function and exchange between the Brd4 and 7SK/HEXIM1 regulatory complexes. Dow EC, Liu H, Rice AP. J Cell Physiol. 2010 Jul . 224(1):84-93. 10.1002/jcp.22096 PubMed 20201073