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Addgene

pEF1a-myc-LRH1(ES)-IRES-neo
(Plasmid #28092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28092 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a-Luc-IRES-Neo
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lrh-1
  • Alt name
    liver receptor homolog-1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nr5a2 (a.k.a. AU020803, D1Ertd308e, Ftf, LRH-1, UF2-H3B)
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF1a-myc-LRH1(ES)-IRES-neo was a gift from Austin Cooney (Addgene plasmid # 28092 ; http://n2t.net/addgene:28092 ; RRID:Addgene_28092)

  • For your References section:

    Canonical Wnt/I. Wagner RT, Xu X, Yi F, Merrill BJ, Cooney AJ. Stem Cells. 2010 Oct . 28(10):1794-804. 10.1002/stem.502 PubMed 20734354
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