Skip to main content
Addgene

pJA304
(Plasmid #28091)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28091 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pDONR P2R-P3
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 2639
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    (Gly)5Ala::mCherry(with introns)::tbb-2 3'UTR
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    748

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP2R (destroyed during cloning)
  • 3′ cloning site attP3 (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer T7, M13R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJA304 was a gift from Julie Ahringer (Addgene plasmid # 28091 ; http://n2t.net/addgene:28091 ; RRID:Addgene_28091)

  • For your References section:

    MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser E, Frokjaer-Jensen C, Jorgensen E, Ahringer J. PLoS One. 2011;6(5):e20082. Epub 2011 May 26. 10.1371/journal.pone.0020082 PubMed 21637852
Related items:
Commonly requested with: