Flag-Rab7A Q86L
(Plasmid #28050)

• Depositing Lab: Qing Zhong
• Publication: Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5137
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rab7A
• Species: H. sapiens (human)
• Insert Size (bp): 623
• Mutation: Q86L
• Entrez Gene: RAB7A (a.k.a. CMT2B, PRO2706, RAB7)
• Tags / Fusion Proteins:
  • 3xFlag (C terminal on insert)
  • 2xHA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry