EGFP-Rubicon delta182
(Plasmid #28041)

• Depositing Lab: Qing Zhong
• Publication: Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 107(45):19338-43.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5078
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rubicon
• Species: H. sapiens (human)
• Insert Size (bp): 2237
• Mutation: delta 1-182aa
• Entrez Gene: RUBCN (a.k.a. KIAA0226, RUBICON, SCAR15)
• Tags / Fusion Proteins:
  • EGFP (N terminal on insert)
  • 3xFlag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry