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Addgene

pTGV-ProK Express SMR5454
(Plasmid #28034)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28034 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    n/a
  • Backbone size (bp) 6583
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pTGV-ProkExpress is pTGV-Light (Addgene plasmid number 28032) with an arabinose-inducible promoter (PBAD) upstream of the multiple cloning site to allow regulatable expression of the transgene. The AraC protein, required for PBAD regulation must be expressed from its normal chromosomal location.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTGV-ProK Express SMR5454 was a gift from Susan Rosenberg (Addgene plasmid # 28034 ; http://n2t.net/addgene:28034 ; RRID:Addgene_28034)
  • For your References section:

    The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM. Gene. 2001 Jul 25. 273(1):97-104. 10.1016/S0378-1119(01)00565-0 PubMed 11483365