pTGV-CAT SMR5453
(Plasmid #28033)

• Purpose: (Empty Backbone)
• Depositing Lab: Susan Rosenberg
• Publication: Gumbiner-Russo et al Gene. 2001 Jul 25. 273(1):97-104.

Backbone

• Vector backbone: n/a
• Backbone size (bp): 7240
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.