pTGV-Express SMR5448
(Plasmid
#28031)
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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28031 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonen/a
- Backbone size (bp) 6610
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTGV-Express SMR5448 was a gift from Susan Rosenberg (Addgene plasmid # 28031 ; http://n2t.net/addgene:28031 ; RRID:Addgene_28031) -
For your References section:
The TGV transgenic vectors for single-copy gene expression from the Escherichia coli chromosome. Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM. Gene. 2001 Jul 25. 273(1):97-104. 10.1016/S0378-1119(01)00565-0 PubMed 11483365