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Addgene

pSL7 SMR1791
(Plasmid #28030)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4361
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MutS, MutL
  • Alt name
    methyl-directed mismatch repair protein
  • Species
    E. coli
  • Entrez Gene
    mutL (a.k.a. b4170, ECK4166)
  • Entrez Gene
    mutS (a.k.a. b2733, ECK2728, ant, fdv, plm)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSL7 contains a ClaI–BglII fragment of pSL6 (carrying mutS), a BamHI–HindIII fragment of pKC31 (conferring Kanamycin resistance), and a HindIII–PstI fragment of pAL51 (carrying mutL)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL7 SMR1791 was a gift from Susan Rosenberg (Addgene plasmid # 28030 ; http://n2t.net/addgene:28030 ; RRID:Addgene_28030)

  • For your References section:

    Mismatch repair protein MutL becomes limiting during stationary-phase mutation. Harris RS, Feng G, Ross KJ, Sidhu R, Thulin C, Longerich S, Szigety SK, Winkler ME, Rosenberg SM. Genes Dev. 1997 Sep 15. 11(18):2426-37. 10.1101/gad.11.18.2426 PubMed 9308969
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