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Addgene

pSL5 SMR1713
(Plasmid #28028)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28028 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4361
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MutL
  • Alt name
    methyl-directed mismatch repair protein
  • Species
    E. coli
  • Entrez Gene
    mutL (a.k.a. b4170, ECK4166)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSL5 contains a PstI–HindIII fragment of pAL51 carrying E. coli mutL and a KanR-conferring HindIII–BamHI fragment of pKC31 replacing digest sites PstI and BamHI of pBR322

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL5 SMR1713 was a gift from Susan Rosenberg (Addgene plasmid # 28028 ; http://n2t.net/addgene:28028 ; RRID:Addgene_28028)

  • For your References section:

    Mismatch repair protein MutL becomes limiting during stationary-phase mutation. Harris RS, Feng G, Ross KJ, Sidhu R, Thulin C, Longerich S, Szigety SK, Winkler ME, Rosenberg SM. Genes Dev. 1997 Sep 15. 11(18):2426-37. 10.1101/gad.11.18.2426 PubMed 9308969
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