AAV-CAG-GFP
(Plasmid #28014)

• Depositing Lab: Karel Svoboda
• Publication: Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
• How this works:
  • Place a request for a quantity of 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: AAV2/1
• Backbone size w/o insert (bp): 5037
• Vector type: AAV ; adeno associate viral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 718

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ageI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: TCGGCTTCTGGCGTGTGACCGG

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 26 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

with ITRs, this vector is easy to have recombination. Check with multiple restriction digestions.