p15aTetT7mut-fuseYFP
(Plasmid
#28013)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 28013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepProlar.A122
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 2500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5alpha pro, 37 degrees
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameEYFP-fuse, T7 RNAP
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SpeciesSynthetic, T7 phage
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Insert Size (bp)3500
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site AvrII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p15aTetT7mut-fuseYFP was a gift from Lingchong You (Addgene plasmid # 28013 ; http://n2t.net/addgene:28013 ; RRID:Addgene_28013) -
For your References section:
Emergent bistability by a growth-modulating positive feedback circuit. Tan C, Marguet P, You L. Nat Chem Biol. 2009 Nov . 5(11):842-8. 10.1038/nchembio.218 PubMed 19801994