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Addgene

pCFPT7
(Plasmid #28012)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28012 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    MC4100Z1 37 degrees C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7 RNAP
  • Species
    T7 phage
  • Insert Size (bp)
    2500

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site AatII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFPT7 was a gift from Lingchong You (Addgene plasmid # 28012 ; http://n2t.net/addgene:28012 ; RRID:Addgene_28012)

  • For your References section:

    Emergent bistability by a growth-modulating positive feedback circuit. Tan C, Marguet P, You L. Nat Chem Biol. 2009 Nov . 5(11):842-8. 10.1038/nchembio.218 PubMed 19801994
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