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Addgene

pCFP
(Plasmid #28011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21 DE3 37 degrees C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP
  • Species
    Synthetic
  • Insert Size (bp)
    1300

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site AatII (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that there are minor discrepancies between Addgene's quality control sequence and the sequence provided by the depositing lab. These discrepancies do not appear to be in critical regions and should not alter the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFP was a gift from Lingchong You (Addgene plasmid # 28011 ; http://n2t.net/addgene:28011 ; RRID:Addgene_28011)

  • For your References section:

    Emergent bistability by a growth-modulating positive feedback circuit. Tan C, Marguet P, You L. Nat Chem Biol. 2009 Nov . 5(11):842-8. 10.1038/nchembio.218 PubMed 19801994
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