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Mfn2-YFP
(Plasmid #28010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YFP-N1
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitofusin 2
  • Alt name
    Mfn2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2282
  • Mutation
    silent S508S
  • GenBank ID
    NM_014874
  • Entrez Gene
    MFN2 (a.k.a. CMT2A, CMT2A2, CMT2A2A, CMT2A2B, CPRP1, HMSN6A, HSG, MARF, MSL)
  • Tag / Fusion Protein
    • YFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer AGGTCTATATAAGCAGAGC
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cloned from Mfn2-GFP: Santel and Fuller, 2001
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Santel, A. and M.T. Fuller, Control of mitochondrial morphology by a human mitofusin. J Cell Sci, 2001. 114(Pt 5): p. 867-74.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mfn2-YFP was a gift from Richard Youle (Addgene plasmid # 28010 ; http://n2t.net/addgene:28010 ; RRID:Addgene_28010)

  • For your References section:

    Spatial and temporal association of Bax with mitochondrial fission sites, Drp1, and Mfn2 during apoptosis. Karbowski M, Lee YJ, Gaume B, Jeong SY, Frank S, Nechushtan A, Santel A, Fuller M, Smith CL, Youle RJ. J Cell Biol. 2002 Dec 23. 159(6):931-8. 10.1083/jcb.200209124 PubMed 12499352
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