E-cadherin-GFP
(Plasmid #28009)

• Depositing Lab: Jennifer Stow
• Publication: Miranda et al J Biol Chem. 2001 Jun 22. 276(25):22565-72.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Geneticin (G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E-cadherin
• Species: H. sapiens (human)
• Insert Size (bp): 2648
• Entrez Gene: CDH1 (a.k.a. Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human Ecadherin gene in pCDNA3.1 we had from Alpha Yap laboratory (University of Queensland in 1998) We inserted GFP at C- terminal of Human Ecadherin in pcDNA3.1
• Articles Citing this Plasmid:
  • 34 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note the EGFP protein sequence in this plasmid is missing the first two amino acids of the standard EGFP sequence (Met-Val) due to the cloning strategy. There is also a Y238I mutation present at the C-terminus of EGFP. These differences do not affect the fluorescent protein properties as described in the associated publication.