pDR-GW AT1.03
(Plasmid #28003)

• Depositing Lab: Wolf Frommer
• Publication: Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Backbone

• Vector backbone: pDRf1GW-ura3
• Backbone size w/o insert (bp): 6866
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AT1.03
• Alt name: ATP sensor Kd: 3.3 mM
• Species: B. Subtilis
• Insert Size (bp): 1836
• Tags / Fusion Proteins:
  • mseCFP (N terminal on insert)
  • mVenus (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: Gateway-based cloning (not destroyed)
• 3′ cloning site: Gateway-based cloning (not destroyed)
• 5′ sequencing primer: pPMA-For 5'-AACAATCGTTAATAATTAATTAATTGG
• 3′ sequencing primer: pHybLex_rev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

AT1.03 is composed of mseCFPdeltaC11, in which 11 C-terminal amino acids were truncated, Bacillus subtilis F0F1 ATP synthase subunit epsilon with
V9T/V42K/F67T/L78T mutations, and cp173-mVenus.