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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 28002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDRf1GW-ura3
- Backbone size w/o insert (bp) 6866
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLII12Pglu-700μδ6
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SpeciesE. Coli
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Insert Size (bp)2316
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Tags
/ Fusion Proteins
- eCFP (N terminal on insert)
- eYFP (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site Gateway-based cloning (not destroyed)
- 3′ cloning site Gateway-based cloning (not destroyed)
- 5′ sequencing primer pPMA-For 5'-AACAATCGTTAATAATTAATTAATTGG
- 3′ sequencing primer pHybLex_rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDR-GW FLII12Pglu-700μδ6 was a gift from Wolf Frommer (Addgene plasmid # 28002 ; http://n2t.net/addgene:28002 ; RRID:Addgene_28002) -
For your References section:
Dynamic analysis of cytosolic glucose and ATP levels in yeast using optical sensors. Bermejo C, Haerizadeh F, Takanaga H, Chermak D, Frommer WB. Biochem J. 2010 Dec 1. 432(2):399-406. 10.1042/BJ20100946 PubMed 20854260