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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 27994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIX (modified)
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 9058
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Vector typeMammalian Expression, Retroviral, RNAi ; Fluorescent reporters
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmid is low conc., low copy. Use Max efficiency DH5alpha for transformations.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTRMPVIR (shRen)
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Alt namePMID: 21131983
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Alt nameGenbank HQ456317
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Alt namemiR30 based shRNAs
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Insert Size (bp)116
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GenBank IDHQ456317
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer miR30seq: 5’-tgtttgaatgaggcttcagtac (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRMPVIR was a gift from Scott Lowe (Addgene plasmid # 27994 ; http://n2t.net/addgene:27994 ; RRID:Addgene_27994) -
For your References section:
Toolkit for evaluating genes required for proliferation and survival using tetracycline-regulated RNAi. Zuber J, McJunkin K, Fellmann C, Dow LE, Taylor MJ, Hannon GJ, Lowe SW. Nat Biotechnol. 2011 Jan . 29(1):79-83. 10.1038/nbt.1720 PubMed 21131983