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Addgene

2.1 kB VEGF-luc
(Plasmid #27988)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 27988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PGL2 Basic
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2.1 kB VEGF
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Srf I (unknown if destroyed)
  • 3′ cloning site EcoR I (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2.1 kB VEGF-luc was a gift from Patricia D'Amore (Addgene plasmid # 27988 ; http://n2t.net/addgene:27988 ; RRID:Addgene_27988)

  • For your References section:

    ErbB2 overexpression in mammary cells upregulates VEGF through the core promoter. Loureiro RM, Maharaj AS, Dankort D, Muller WJ, D'Amore PA. Biochem Biophys Res Commun. 2005 Jan 14. 326(2):455-65. 10.1016/j.bbrc.2004.11.053 PubMed 15582599
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